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Image Search Results
Journal: Nutrients
Article Title: Dietary Fiber Intake Improves Osteoporosis Caused by Chronic Lead Exposure by Restoring the Gut-Bone Axis.
doi: 10.3390/nu17091513
Figure Lengend Snippet: Figure 3. DF restored intestinal structure and Treg cells disrupted by Pb exposure. (A) Hematoxylin and eosin-stained sections (n = 6–10) at a 200 µm scale, with insets showing 100 µm-magnified regions. Annotations: inflammatory infiltrates (red arrows), goblet cells (blue arrows), intestinal crypts (yellow arrows). (B) Studies on intestinal morphology involved the assessment of cumulative scores encompassing inflammatory infiltration, crypt damage, and goblet cell impairment. (C,D) The mRNA expression of the ZO-1 and Occludin gene in colon tissue (n = 6–10). (E–G) The protein expression of the ZO-1 and Occludin gene in colon tissue (n = 3). (H–J) Representative flow cytometry image of Treg cell subpopulations in the colon. (K) Percentage of Treg cell subsets in CD4 + T cells in mice colon (n = 6–10). (L–N) Representative flow cytometry images of Treg cell subpopulations in the bone. (O) Percentage of Treg cell subsets in CD4 + T cells in mice bone (n = 6–10). Data are presented as mean ± SD. * p < 0.05, ** p< 0.01, *** p < 0.001, and **** p < 0.0001 (Dunnett multiple-comparison test).
Article Snippet: The primary antibodies involved included
Techniques: Staining, Expressing, Flow Cytometry, Comparison
Journal: World Journal of Stem Cells
Article Title: Transplanting embryonic stem cells onto damaged human corneal endothelium
doi: 10.4252/wjsc.v9.i8.127
Figure Lengend Snippet: Endothelial parts of three corneal sections: A-C, D-F, and G-I. A: Nuclei through a 4‘,6-diamidino-2-phenylindole (DAPI) filter; B: The same cells through a fluorescein isothiocyanate (FITC) filter [green fluorescent protein (GFP) fluorescence]; C: Expression of the tight junction protein Zona Occludens protein 1 (ZO-1) [tetramethylrhodamine (TRITC) filter]; D: Nuclei seen through a DAPI filter; E: GFP expression (using an FITC filter); F: Expression of sodium potassium ATPase (NaKATPase) (using a TRITC filter); G: Nuclei seen through a DAPI filter; H: GFP expression (FITC filter); I: Expression of the cornea-related protein paired box 6 (PAX-6).
Article Snippet: The secondary antibodies used were CK3 [mouse monoclonal antibody, AE5 (ab77869); Abcam, Cambridge, United Kingdom], a marker for corneal epithelial cells (Abcam); paired box 6 (PAX-6) [mouse monoclonal antibody, AD2.38 (ab78545), Abcam], a marker for a transcription factor important in the development of the eye; octamer-binding transcription factor 3/4 (OCT-3/4) [mouse monoclonal antibody, C-10 (sc-5279), Santa Cruz Biotechnology, Inc., Santa Cruz, CA, United States], a marker for embryonic stem cell activity; sodium potassium adenosine triphosphatase (NaKATPase) [rabbit monoclonal antibody (ab58475), Abcam], a marker for NaKATPase pump function; and
Techniques: Fluorescence, Expressing